GWS playmaker Josh Kelly will miss Saturday's AFL semi-final against an unchanged Collingwood because of a knee injury.
And Hawthorn have lost midfielder Jaeger O'Meara (knee) as well as Ben Stratton (hamstring) for Friday night's semi-final against the same Melbourne side that knocked out Geelong.
The Hawks have also dumped Jon Ceglar while recalling Taylor Duryea, David Mirra, and Ryan Schoenmakers.
While Stratton's absence was known after he was hurt last week, O'Meara's injury is a fresh blow.
Hawthorn's football manager Graham Wright says O'Meara copped a knock to his left knee during the qualifying final loss to Richmond.
"We gave him every opportunity to get himself right for the semi-final but he just hasn't pulled up in time," Wright said.
"He's an important player so it's certainly disappointing."
Meanwhile, Giants coach Lean Cameron retains hope Kelly, who has been replaced by veteran Ryan Griffen, will be available next week if GWS beat the Magpies.
The Giants recalled Lachie Keefe to play against his former club with Harry Perryman dropped from the side which trounced Sydney in last Saturday's elimination final.
Collingwood named the same team which slipped to 16-point loss to West Coast last Saturday night.
GWS boss Cameron said star midfielder Kelly was given every chance to prove his fitness.
"We gave him until Thursday to see how he would be progressing and he's a good chance to play next week, but we're not going to take him in against Collingwood," he said.
Veteran Griffen, 32, hasn't played since straining a hamstring in round 22.
"We toyed with the prospect of bringing him in last week," Cameron said.
"He has had a good three or four weeks (training) and he has been really good for us this year.
"He is a finals player, he knows what that MCG atmosphere is going to be like and he'll come in and play his role."
Cameron was adamant his players wont be fazed by a pro-Magpie MCG crowd of around 85,000 spectators.
"We know we're going to play against not only a fantastic footy team but also a huge crowd," he said.
"We had that last year against Richmond (in a preliminary final) and we fell short but I felt we handled ourselves pretty well.
"It wasn't the crowd that got us, it was the footy that we need to play a little bit better.
"Finals footy is about backing up and we know that if we don't back up, then Collingwood will beat us, it's as simple as that."